Pulsed-Field Gel Electrophoresis of Degradation-Sensitive DNAs from Clostridium difficile PCR Ribotype 1 Strains

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Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile.

Clostridium difficile is now recognized as the major agent responsible for nosocomial diarrhea in adults. Among the genotyping methods available, arbitrarily primed PCR (AP-PCR), PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) have been widely used for investigating outbreaks of C. difficile infections. However, the comparative typing ability, reproducibility, discriminatory power, ...

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Pulsed-field gel electrophoresis.

DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 2000

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.38.7.2791-2792.2000